Importance of Guide-RNA Design Using Big Data in Insect Molecular Breeding Research 


Vol. 45,  No. 8, pp. 1447-1451, Aug.  2020
10.7840/kics.2020.45.8.1447


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  Abstract

In this paper, we analyzed the necessity and effect of using genome big data when studying molecular breeding for various species. A guide RNA for gene editing was selected and produced by inputting the sequence of the region that needs to be edited into a genome data base web server and cross-verifying it with genome big data. The efficiency was verified by making the mutant by applying the prepared guide RNA to the silkworm, and the accuracy of the target sequence was verified through chromosomal sequencing of the mutant. As a result, it was confirmed that the guide RNA produced through the CRISPRdirect server based on the big data enables specific gene editing by exhibiting specific reactivity to the target site.

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  Cite this article

[IEEE Style]

C. Y. Jeong, J. W. Hong, J. H. Yu, S. Kim, S. K. Kang, S. Kim, N. Kim, K. Y. Kim, J. W. Park, "Importance of Guide-RNA Design Using Big Data in Insect Molecular Breeding Research," The Journal of Korean Institute of Communications and Information Sciences, vol. 45, no. 8, pp. 1447-1451, 2020. DOI: 10.7840/kics.2020.45.8.1447.

[ACM Style]

Chan Young Jeong, Jeong Won Hong, Jeong Hee Yu, Su-Bae Kim, Sang Kuk Kang, Seong-Wan Kim, Nam-Suk Kim, Kee Young Kim, and Jong Woo Park. 2020. Importance of Guide-RNA Design Using Big Data in Insect Molecular Breeding Research. The Journal of Korean Institute of Communications and Information Sciences, 45, 8, (2020), 1447-1451. DOI: 10.7840/kics.2020.45.8.1447.

[KICS Style]

Chan Young Jeong, Jeong Won Hong, Jeong Hee Yu, Su-Bae Kim, Sang Kuk Kang, Seong-Wan Kim, Nam-Suk Kim, Kee Young Kim, Jong Woo Park, "Importance of Guide-RNA Design Using Big Data in Insect Molecular Breeding Research," The Journal of Korean Institute of Communications and Information Sciences, vol. 45, no. 8, pp. 1447-1451, 8. 2020. (https://doi.org/10.7840/kics.2020.45.8.1447)

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pISSN : 1226 - 4717
eISSN : 2287 - 3880